This week in lab we were able to perform out own susceptibility tests. These tests included an agar disk diffusion on Staph epidermidis, an MIC combo plate, E test, CHROMagar, and Nitrocefin disk for beta-lactamase. For the agar diffusion, the plates were streaked and disks were placed and incubated overnight. The next day the zone was measured around each disk. Using given values, susceptibility was determined.
Then, a Microscan gram negative MIC panel was done. The pan is innoculated and incubated to be read the following day. Each well is read for growth or no growth, and each MIC is circled on the report sheet.
The CHROMAgar was streaked with the specimen swab and icubated. The plates were then read and interpreted. If mauve colonies were present, it is positive.
Lastly, the Nitrocefin disk test was performed. The red color seen below indicates a positive result and means the organism is resistant to penacillin and cephalosporin.
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